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e coli s2060 addgene  (Addgene inc)


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    Addgene inc e coli s2060 addgene
    Figure 2. Development and validation of a prime editing PACE selection (A) Schematic of PE-PACE selection circuit. Upon infection of <t>E.</t> <t>coli</t> by selection phage (blue), the NpuN intein and NpuC intein (pink) mediate reconstitution of the PE2 prime editor (purple and pink), which engages a pegRNA (dark green) and corrects a frameshift in T7 RNAP (orange) via PE. Functional T7 RNAP then transcribes gIII (light green), which enables SP propagation. (B) Phage replication levels from overnight propagation of empty phage (red), NpuC-PE2-RT phage (purple), and T7-RNAP phage (green) in PE-PACE host cells before pegRNA optimization.
    E Coli S2060 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+s2060+addgene/pm37657419-436-22-25?v=Addgene+inc
    Average 92 stars, based on 13 article reviews
    e coli s2060 addgene - by Bioz Stars, 2026-07
    92/100 stars

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    1) Product Images from "Phage-assisted evolution and protein engineering yield compact, efficient prime editors."

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors.

    Journal: Cell

    doi: 10.1016/j.cell.2023.07.039

    Figure 2. Development and validation of a prime editing PACE selection (A) Schematic of PE-PACE selection circuit. Upon infection of E. coli by selection phage (blue), the NpuN intein and NpuC intein (pink) mediate reconstitution of the PE2 prime editor (purple and pink), which engages a pegRNA (dark green) and corrects a frameshift in T7 RNAP (orange) via PE. Functional T7 RNAP then transcribes gIII (light green), which enables SP propagation. (B) Phage replication levels from overnight propagation of empty phage (red), NpuC-PE2-RT phage (purple), and T7-RNAP phage (green) in PE-PACE host cells before pegRNA optimization.
    Figure Legend Snippet: Figure 2. Development and validation of a prime editing PACE selection (A) Schematic of PE-PACE selection circuit. Upon infection of E. coli by selection phage (blue), the NpuN intein and NpuC intein (pink) mediate reconstitution of the PE2 prime editor (purple and pink), which engages a pegRNA (dark green) and corrects a frameshift in T7 RNAP (orange) via PE. Functional T7 RNAP then transcribes gIII (light green), which enables SP propagation. (B) Phage replication levels from overnight propagation of empty phage (red), NpuC-PE2-RT phage (purple), and T7-RNAP phage (green) in PE-PACE host cells before pegRNA optimization.

    Techniques Used: Biomarker Discovery, Selection, Infection, Functional Assay



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    e coli s2060 addgene  (Addgene inc)
    92
    Addgene inc e coli s2060 addgene
    Figure 2. Development and validation of a prime editing PACE selection (A) Schematic of PE-PACE selection circuit. Upon infection of <t>E.</t> <t>coli</t> by selection phage (blue), the NpuN intein and NpuC intein (pink) mediate reconstitution of the PE2 prime editor (purple and pink), which engages a pegRNA (dark green) and corrects a frameshift in T7 RNAP (orange) via PE. Functional T7 RNAP then transcribes gIII (light green), which enables SP propagation. (B) Phage replication levels from overnight propagation of empty phage (red), NpuC-PE2-RT phage (purple), and T7-RNAP phage (green) in PE-PACE host cells before pegRNA optimization.
    E Coli S2060 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+s2060+addgene/pm37657419-436-22-25?v=Addgene+inc
    Average 92 stars, based on 1 article reviews
    e coli s2060 addgene - by Bioz Stars, 2026-07
    92/100 stars
    		  Buy from Supplier

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    Figure 2. Development and validation of a prime editing PACE selection (A) Schematic of PE-PACE selection circuit. Upon infection of E. coli by selection phage (blue), the NpuN intein and NpuC intein (pink) mediate reconstitution of the PE2 prime editor (purple and pink), which engages a pegRNA (dark green) and corrects a frameshift in T7 RNAP (orange) via PE. Functional T7 RNAP then transcribes gIII (light green), which enables SP propagation. (B) Phage replication levels from overnight propagation of empty phage (red), NpuC-PE2-RT phage (purple), and T7-RNAP phage (green) in PE-PACE host cells before pegRNA optimization.

    Journal: Cell

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors.

    doi: 10.1016/j.cell.2023.07.039

    Figure Lengend Snippet: Figure 2. Development and validation of a prime editing PACE selection (A) Schematic of PE-PACE selection circuit. Upon infection of E. coli by selection phage (blue), the NpuN intein and NpuC intein (pink) mediate reconstitution of the PE2 prime editor (purple and pink), which engages a pegRNA (dark green) and corrects a frameshift in T7 RNAP (orange) via PE. Functional T7 RNAP then transcribes gIII (light green), which enables SP propagation. (B) Phage replication levels from overnight propagation of empty phage (red), NpuC-PE2-RT phage (purple), and T7-RNAP phage (green) in PE-PACE host cells before pegRNA optimization.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli Thermo Fisher Scientific Cat#C862003 E. coli S2060 Addgene #105064 Chemicals, peptides, and recombinant proteins BsaI-HFv2 New England BioLabs Cat#R3733S LguI (SapI) Thermo Fisher Scientific Cat#ER1932 T4 DNA Ligase New England BioLabs Cat#M0202S NEBuilder HiFi DNA assembly master mix New England BioLabs Cat#E2621S Dimethyl sulfoxide Sigma-Aldrich Cat#D8418-50ML Poly(ethylene glycol) 3350 Sigma-Aldrich Cat#P4338-500G DNaseI (Rnase-free) New England BioLabs Cat#M0303 Magnesium chloride solution Sigma-Aldrich Cat#M1028-10X1ML Carbenicillin Gold Biotechnology Cat#C-103 Chloramphenicol Gold Biotechnology Cat#C-105 Tetracycline Gold Biotechnology Cat#T-101 Streptomycin Gold Biotechnology Cat#S-150 L-arabinose Gold Biotechnology Cat#A-300 Glucose Sigma-Aldrich Cat#G7021 Bluo-gal Gold Biotechnology Cat#B-673-10 dNTPs New England BioLabs Cat#N0447S Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 TrypLE Thermo Fisher Scientific Cat#12605010 Proteinase K, recombinant, PCR grade Thermo Fisher Scientific Cat#11668019 SDS (10% wt/vol) Thermo Fisher Scientific Cat#15553027 DNAdvance Kit Beckman Coulter Cat#A48705 AMPure XP Beckman Coulter Cat#B23318 CleanCap Reagent AG TriLink BioTechnologies Cat#N-7113 N1 -Methylpseudouridine50 -Triphosphate TriLink BioTechnologies Cat#N-1081 LiCl Precipitation Solution (7.5 M) Thermo Fisher Scientific Cat#AM9480 DMEM, high glucose, GlutaMAX supplement Thermo Fisher Scientific Cat#10566016 Fetal bovine serum Thermo Fisher Scientific Cat#16000044 L-Glutamine Corning Cat#25-005-Cl Penicillin-Streptomycin Thermo Fisher Scientific Cat#15070063 GlutaMAX supplement Thermo Fisher Scientific Cat#35050061 N-acetyl-L-cysteine Sigma-Aldrich Cat#A7250-100G Human AB Serum Valley Biomedical Cat#HP1022HI Recombinant Human IL-2 Peprotech Cat#200-02 Lymphoprep density gradient medium STEMCELL Technologies Cat#07801 (Continued on next page) Cell 186, 3983–4002.e1–e13, August 31, 2023 e1

    Techniques: Biomarker Discovery, Selection, Infection, Functional Assay